Abdominal Pain CLEAR

Investigations Guide

Laboratory tests and imaging with normal ranges, clinical significance, and condition-specific ordering

20

Blood Tests

2

Urine Tests

9

Imaging

5

Procedures

Complete Blood Count

bloodimmediate
Normal Range: WBC 4,500-11,000/mm³
Abnormal: Leukocytosis > 11,000; > 18,000 suggests moderate-severe disease

Confirms inflammation; high WBC suggests complications

Acute CholecystitisAcute PancreatitisPeptic Ulcer DiseaseIrritable Bowel Syndrome (IBS)Small Bowel ObstructionFunctional DyspepsiaAbdominal Aortic Aneurysm (AAA) RuptureAcute Mesenteric IschemiaDyspepsia — Approach to the PatientAcute Abdominal Pain — General Approach

C-Reactive Protein

bloodimmediate
Normal Range: < 5 mg/L
Abnormal: Elevated CRP

Acute phase reactant; correlates with severity

Acute Cholecystitis

Liver Function Tests

bloodimmediate
Normal Range: ALT 7-56 U/L, AST 10-40 U/L, ALP 44-147 U/L
Abnormal: Elevated bilirubin (up to 4 mg/dL), elevated ALP/GGT

Elevation suggests choledocholithiasis

Acute CholecystitisAcute PancreatitisAcute Abdominal Pain — General Approach

Lipase

bloodimmediate
Normal Range: 0-160 U/L
Abnormal: Elevated > 3x upper limit

If elevated, consider gallstone pancreatitis

Acute CholecystitisAcute Abdominal Pain — General Approach

RUQ Ultrasound

imagingimmediate
Abnormal: Gallstones, gallbladder wall thickening > 3mm, pericholecystic fluid, sonographic Murphy's sign

First-line imaging — sensitivity 81%, specificity 83%

Acute CholecystitisAcute Pancreatitis

HIDA Scan (Cholescintigraphy)

imagingurgent
Abnormal: Non-visualization of gallbladder at 4 hours

Gold standard if ultrasound equivocal — sensitivity 97%, specificity 90%

Acute Cholecystitis

Serum Lipase

bloodimmediate
Normal Range: 0-160 U/L
Abnormal: > 3x upper limit of normal (> 480 U/L)

Diagnostic criterion — sensitivity 85-100%

Acute Pancreatitis

Serum Amylase

bloodimmediate
Normal Range: 30-110 U/L
Abnormal: > 3x upper limit of normal

Less specific than lipase; normalizes faster

Acute Pancreatitis

Basic Metabolic Panel

bloodimmediate
Normal Range: BUN 7-20 mg/dL, Cr 0.7-1.3 mg/dL
Abnormal: Elevated BUN, elevated glucose, low calcium

BUN > 20 at admission predicts mortality

Acute PancreatitisSmall Bowel ObstructionAcute Abdominal Pain — General Approach

CT Abdomen with Contrast

imagingurgent
Abnormal: Pancreatic enlargement, peripancreatic fluid, necrosis, pseudocyst

Not needed for diagnosis if clinical + lipase clear. Obtain at 72-96 hours if no improvement

Acute PancreatitisSmall Bowel Obstruction

H. pylori Testing

bloodroutine
Normal Range: Negative
Abnormal: Positive urea breath test, stool antigen, or serology

Identifies treatable cause; urea breath test preferred

Peptic Ulcer DiseaseDyspepsia — Approach to the Patient

Upper GI Endoscopy (EGD)

procedureurgent
Abnormal: Mucosal defect, active bleeding, malignant features

Gold standard for diagnosis; allows biopsy and treatment

Peptic Ulcer Disease

Gastric Biopsy

procedureurgent
Abnormal: H. pylori organisms, dysplasia, malignancy

All gastric ulcers should be biopsied to exclude malignancy

Peptic Ulcer Disease

CRP or ESR

bloodroutine
Normal Range: CRP < 5 mg/L
Abnormal: Elevated suggests IBD, not IBS

Helps distinguish IBS from IBD

Irritable Bowel Syndrome (IBS)

Celiac Serology (TTG-IgA)

bloodroutine
Normal Range: Negative
Abnormal: Positive TTG antibodies

Celiac disease mimics IBS — must exclude

Irritable Bowel Syndrome (IBS)

Fecal Calprotectin

bloodroutine
Normal Range: < 50 µg/g
Abnormal: > 50 µg/g suggests inflammation (IBD)

Best non-invasive test to distinguish IBS from IBD in patients < 45

Irritable Bowel Syndrome (IBS)

Thyroid Function Tests

bloodroutine
Normal Range: TSH 0.4-4.0 mIU/L
Abnormal: Hyper/hypothyroidism

Thyroid disease can mimic IBS symptoms

Irritable Bowel Syndrome (IBS)

Abdominal X-ray (Erect + Supine)

imagingimmediate
Abnormal: Dilated small bowel loops (> 3 cm), air-fluid levels, absence of colonic gas

Sensitivity 70-80%; multiple air-fluid levels diagnostic

Small Bowel ObstructionAcute Abdominal Pain — General Approach

Serum Lactate

bloodimmediate
Normal Range: < 2 mmol/L
Abnormal: Elevated > 2 mmol/L

Elevated lactate suggests bowel ischemia/strangulation

Small Bowel ObstructionAcute Mesenteric Ischemia

H. pylori Test

bloodroutine
Normal Range: Negative
Abnormal: Positive

Test and treat strategy for uninvestigated dyspepsia in patients < 60

Functional Dyspepsia

Upper GI Endoscopy

procedureroutine
Abnormal: Normal (required for diagnosis)

Indicated if age > 60, alarm features, or treatment failure

Functional DyspepsiaGastroparesisDyspepsia — Approach to the Patient

Gastric Emptying Scintigraphy

imagingroutine
Normal Range: < 10% retention at 4 hours
Abnormal: > 10% retention at 4 hours (> 60% at 2 hours)

Gold standard diagnostic test

Gastroparesis

HbA1c

bloodroutine
Normal Range: < 6.5%
Abnormal: Elevated (poor glycemic control)

Hyperglycemia worsens gastric emptying

Gastroparesis

CT Angiography

imagingimmediate
Abnormal: Aneurysm with retroperitoneal hemorrhage, contrast extravasation

Definitive diagnosis in hemodynamically STABLE patients only

Abdominal Aortic Aneurysm (AAA) Rupture

Bedside Ultrasound (FAST)

imagingimmediate
Abnormal: Aortic diameter > 3 cm, free fluid

Rapid bedside assessment; can confirm AAA presence

Abdominal Aortic Aneurysm (AAA) Rupture

Type and Crossmatch

bloodimmediate
Abnormal: N/A — preparation for transfusion

Prepare 6-10 units pRBC, activate massive transfusion protocol

Abdominal Aortic Aneurysm (AAA) Rupture

CT Angiography (Mesenteric)

imagingimmediate
Abnormal: Arterial occlusion, venous thrombosis, bowel wall thickening, pneumatosis, portal venous gas

Gold standard — sensitivity > 95% for arterial occlusion

Acute Mesenteric Ischemia

Arterial Blood Gas

bloodimmediate
Normal Range: pH 7.35-7.45
Abnormal: Metabolic acidosis (low pH, low bicarbonate, elevated lactate)

Metabolic acidosis indicates tissue ischemia

Acute Mesenteric Ischemia

D-Dimer

bloodimmediate
Normal Range: < 500 ng/mL
Abnormal: Elevated

Sensitive but not specific; normal D-dimer has high negative predictive value

Acute Mesenteric Ischemia

Urea Breath Test (UBT)

procedureroutine
Normal Range: Negative
Abnormal: Positive (labeled CO2 detected)

Preferred non-invasive test — sensitivity/specificity > 95%. Must stop PPI 2 weeks before.

Helicobacter pylori Infection

Stool Antigen Test

bloodroutine
Normal Range: Negative
Abnormal: Positive

Alternative to UBT — sensitivity/specificity > 90%. Must stop PPI 2 weeks before.

Helicobacter pylori Infection

Gastric Biopsy (at endoscopy)

procedureroutine
Abnormal: H. pylori organisms on histology or rapid urease test

Gold standard if endoscopy performed

Helicobacter pylori Infection

H. pylori Serology (IgG)

bloodroutine
Normal Range: Negative
Abnormal: Positive IgG antibodies

Cannot distinguish active from past infection; NOT for eradication confirmation

Helicobacter pylori Infection

Urinalysis

urineimmediate
Normal Range: No blood, WBC, nitrites
Abnormal: Hematuria, pyuria, nitrites

UTI, nephrolithiasis

Acute Abdominal Pain — General Approach

Urine Pregnancy Test

urineimmediate
Normal Range: Negative
Abnormal: Positive

MUST exclude ectopic pregnancy in reproductive-age women

Acute Abdominal Pain — General Approach

CT Abdomen/Pelvis with Contrast

imagingimmediate
Abnormal: Varies by condition

Most useful single imaging study for acute abdomen in adults

Acute Abdominal Pain — General Approach